Liquid Chromatographic Assay of Plasma Hemoglobin Based on Oxidative Quenching of Serotonin by Pseudoperoxidase Effect of Hemoglobin

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概要

• 論文の詳細を見る

• A high-performance liquid chromatographic method for the determination of hemoglobin is described. The method uses the pseudoperoxidase effect of hemoglobin in the presence of hydrogen peroxide and is based on the oxidative quenching of the native fluorescence of serotonin. The determination range for hemoglobin was between 3 and 100 mg/dl. The intra-assay coefficient of variation was 2.04% or less, with a mean recovery of 102.0% at hemoglobin concentrations of 17.28,43.20,and 69.12 mg/dl. A close correlation was found between the results of this method and the leuco crystal violet method.

• 公益社団法人日本薬学会の論文
• 1988-08-25

著者

• 高柳 美行

  愛知県赤十字血液センター

• 小久保 幸雄

  愛知県赤十字血液センター

• 八代 有

  Faculty of Pharmaceutical Sciences Nagoya City University

• 後藤 鉦二

  愛知県赤十字血液センター

• 八代 有

  名古屋市立大学薬学部

• 高柳 美行

  Aichi Prefecture Red Cross Blood Center

• 後藤 鉦二

  Aichi Prefecture Red Cross Blood Center

• 小久保 幸雄

  Aichi Prefecture Red Cross Blood Center

• 八代 有

  名古屋市立大学

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