HPLC-蛍光検出法による過酸化水素の定量およびその応用としての血漿L-(+)-乳酸測定

概要

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A sensitive and specific method for the determination of hydrogen peroxide by high performance liquid chromatography is developed. It is based on elimination of the native fluorescence of 5-hydroxytryptamine by an oxidative reaction between 5-hydroxytryptamine and hydrogen peroxide in the presence of peroxidase. Under the described conditions, hydrogen peroxide was determined in the concentration range of 0.5-10μM; the within-assay coefficients of variation (C.V.s) were 2.41% or less, with a mean recovery of 103.2% against additional hydrogen peroxide in the samples. This system can be utilized for the determination of plasma L-(+)-lactate using L-(+)-lactate oxidase. The detectable range of L-(+)-lactate was 0.5-8.0mM; C.V.s were 6.76% or less.
日本医療薬学会の論文
1989-06-20