Synthesis of a 37-Residue Peptide Amide Corresponding to the Entire Amino Acid Sequence of α-Form of Rat Calcitonin Gene-Related Peptide (α-rCGRP)(Organic,Chemical)

概要

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The heptatriacontapeptide amide corresponding to the entire amino acid sequence of α-form of rat calcitonin gene-related peptide (α-rCGRP) was synthesized by the conventional solution method. All protecting groups employed were removed by treatment with 1 M trifluoromethane-sulfonic acid-thioanisole-trifluoroacetic acid, and the deprotected peptide was subjected to air-oxidation to form the intramolecular disulfide bond. After purification by gel-filtration, on Sephadex G-50, followed by reversed-phase high performance liquid chromatography, al highly purified sample of synthetic α-rCGRP was obtained. In terms of suppression of bone ^<45>Ca-release stimulated by synthetic human parathyroid hormone (1-34), synthetic α-rCGRP was as active as synthetic human CGRP.
社団法人日本薬学会の論文
1987-01-25