Anabolic Effects of Bee Pollen Cistus ladaniferus Extract on Bone Components in the Femoral-Diaphyseal and -Metaphyseal Tissues of Rats in Vitro and in Vivo
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概要
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The effects of bee pollen extract on bone components in the femoral-diaphyseal (cortical bone) and -metaphyseal (trabecular bone) tissues of rats in vitro and in vivo were investigated. Bone tissues were cultured for 48 hr in serum-free Dulbecco's modified Eagle's medium containing either vehicle or water- or ethanol-solubilized extracts (10, 100, or 1000μg/ml of medium) obtained from the bee pollen of Cistus ladaniferus. Calcium content in the femoral-diaphyseal or -metaphyseal tissues was significantly increased in the presence of water-solubilized extract (100 or 1000μg/ml) and ethanol-solubilized extract (1000μg/ml). An increase was also observed in the presence of water-solubilized extract (100μg/ml) obtained from Fagopyrum esculentum, Camellia sinesis, or Brassica napus L. Alkaline phosphatase activity and DNA content in the femoral-diaphyseal or -metaphyseal tissues in vitro were significantly increased in the presence of water-solubilized extract (100 or 1000μg/ml) obtained from the bee pollen. The effects of the bee pollen extract (100μg/ml) in increasing bone components were completely inhibited in the presence of cycloheximide (10^<-6> M), an inhibitor of protein synthesis, in vitro. Moreover, the calcium content and alkaline phosphatase activity in the femoral-diaphyseal or -metaphyseal tissues were significantly increased by the oral administration of water-solubilized extracts (5 or 10mg/100g body weight) obtained from the bee pollen of Cistus ladaniferus once daily for 7 days. The DNA content in the diaphyseal or metaphyseal tissues was significantly increased by the oral administration of water-solubilized extract (10mg/100g) of bee pollen cistus. The dose of 1.0mg/100g caused a significant increase in the diaphyseal and metaphyseal alkaline phosphatase activity or the metaphyseal DNA content in vivo. This study demonstrates that the extract of bee pollen has an anabolic effect on bone components in rats in vitro and in vivo.
- 2006-02-01
著者
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ISHIYAMA Kaori
Institute for Bee Products & Health Science, Yamada Apiculture Center Inc.
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Uchiyama Satoshi
Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, Unive
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Ishiyama Kaori
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Yamaguchi Masayoshi
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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HASHIMOTO Ken
Institute for Bee Products and Health Science, Yamada Apiculture Center, Inc.
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Yamaguchi M
Univ. Shizuoka Sizuoka Jpn
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Hamamoto Reiko
Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, Unive
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Uchiyama Satoshi
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Hashimoto Ken
Institute For Bee Products & Health Science Yamada Apiculture Center Inc.
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Hamamoto Reiko
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Uchiyama Satoshi
Laboratory Of Biology Faculty Of Education And Human Sciences Niigata University
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Yamaguchi Masayoshi
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Yamaguchi Masayoshi
Laboratories Of Metabolism And Endocrinology School Of Food And Nutritional Sciences The University
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