Synergistic Effect of β-Cryptoxanthin and Zinc Sulfate on the Bone Component in Rat Femoral Tissues in Vitro : The Unique Anabolic Effect with Zinc(Pharmacology)
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概要
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The effect of the combination of β-cryptoxanthin and zinc sulfate (zinc) on bone components in the femoral-diaphyseal and -metaphyseal tissues of young rats in vitro was investigated. Bone tissues were cultured for 48h in a serum-free Dulbecco's modified Eagle's medium containing either vehicle, β-cryptoxanthin (10^<-9>-10^<-7>M) or zinc sulfate (10^<-6>-10^<-4>M). The presence of β-cryptoxanthin (10^<-9>M) or zinc (10^<-6>M) did not have a significant effect on calcium content in the femoral-diaphyseal or-metaphyseal tissues. However, culture which combined β-cryptoxanthin (10^<-9>M) and zinc (10^<-6>M) caused a significant increase in calcium content in the femoral-diaphyseal and -metaphyseal tissues. Such an effect was not observed by the combination of β-cryptoxanthin (10^<-9>M) plus genistein (10^<-6>M) or menaquinone-7 (10^<-6>M), or zinc (10^<-6>M) plus genistein (10^<-6>M) or menaquinone-7 (10^<-6>M). Also, the combination of β-cryptoxanthin (10^<-9>M) plus zinc (10^<-6>M) caused a remarkable increase in alkaline phosphatase activity and deoxyribonucleic acid (DNA) in the femoral-diaphyseal and -metaphyseal tissues, while their application alone did not have an effect on the enzyme activity or DNA content in the femoral tissues. The effect of the combination of β-cryptoxanthin (10^<-9>M) plus zinc (10^<-6>M) in increasing calcium content, alkaline phosphatase activity, and DNA content in the femoral-diaphyseal and -metaphyseal tissues was completely prevented in the presence of cycloheximide (10^<-6>M), an inhibitor of protein synthesis, or 5, 6-dichloro-1-β-D-ribofuranosylbenzimidazole (DBR), an inhibitor of transcriptional activity. This study demonstrates that the combination of β-cryptoxanthin and zinc at a lower concentration has a synergistic effect on bone components in vitro.
- 公益社団法人日本薬学会の論文
- 2005-11-01
著者
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ISHIYAMA Kaori
Institute for Bee Products & Health Science, Yamada Apiculture Center Inc.
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Uchiyama Satoshi
Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, Unive
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Ishiyama Kaori
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Yamaguchi Masayoshi
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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HASHIMOTO Ken
Institute for Bee Products and Health Science, Yamada Apiculture Center, Inc.
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Yamaguchi M
Univ. Shizuoka Sizuoka Jpn
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Uchiyama Satoshi
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Hashimoto Ken
Institute For Bee Products & Health Science Yamada Apiculture Center Inc.
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Uchiyama Satoshi
Laboratory Of Biology Faculty Of Education And Human Sciences Niigata University
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Yamaguchi Masayoshi
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Yamaguchi Masayoshi
Laboratories Of Metabolism And Endocrinology School Of Food And Nutritional Sciences The University
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