Suppressive Effect of Genistein on Rat Bone Osteoclasts : Apoptosis Is Induced through Ca^<2+> Signaling
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概要
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The effect of genistein on osteoclase-like multinucleated cells (MNCs) from rat femoral tissues was investigated. When rat marrow cells were cultured for 7 d in a medium containing either vehicle, parathyroid hormone (10^<-8> M) or prostaglandine E_2 (10^<-6> M), the formation of tartrate-resistant acid phosphatase (TRACP)-positive MNCs was significantly increased. This increase was markedly weakened by the presence of genistein (10^<-6> or 10^<-5> M). The bone cells isolated from rat femoral tissues were cultured for 48 h in an α-minimal essential medium (5% fetal bovine serum) containing either vehicle or genistein (10^<-8> to 10^<-5> M). Osteoclasts were estimated by staining for TRACP, a marker enzyme of osteoclasts. The presence of genistein caused a significant decrease in the number of osteoclasts. Such a decrease was also seen in the presence of calcitonin (10^<-10> to 10^<-8> M), dibutyryl cyclic adenosine 5'-monophosphate (DcAMP; 10^<-6> and 10^<-5> M), calcium chloride (10^<-4> and 10^<-3> M) or daidzein (10^<-7> to 10^<-5> M). The suppressive effect of genistein (10^<-5> M) was not further enhanced in the presence of calcitonin (10^<-8> M), DcAMP (10^<-5> M), or calcium chloride (10^<-3> M), and was completely abolished by the presence of dibucaine (10^<-6> M) or staurosporine (10^<-7> M), both of which are inhibitors of Ca^<2+>-dependent protein kinases. Ca^<2+> ionophore (A23187; 10^<-6> M) induced a remarkable decrease in the number of osteoclasts in the absence or presence of genistein (10^<-5> M) or calcium chloride (10^<-3> M). The present study demonstrates that genistein has a direct suppressive effect on osteoclasts in vitro, suggesting that the isoflavone may induce apoptosis which is mediated through the pathway of intracellular Ca^<2+> signaling.
- 社団法人日本薬学会の論文
- 1999-08-15
著者
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Gao Ying
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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YAMAGUCHI Masayoshi
Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, Unive
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Yamaguchi Masayoshi
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Yamaguchi Masayoshi
Laboratories Of Metabolism And Endocrinology School Of Food And Nutritional Sciences The University
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Yamaguchi Masayoshi
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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GAO Ying
Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka
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