Inhibitory Effect of Marine Alga Sargassum horneri Extract on Bone Resorption in Tissue Culture in Vitro

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The effect of marine alga Sargassum horneri extracts on bone resorption in vitro was investigated. Femoral-diaphyseal and -metaphyseal tissues obtained from normal rats were cultured for 48 hr in Dulbecco's modified Eagle's medium (high glucose, 4.5%) supplemented with antibiotics and bovine serum albumin. The experimental cultures contained water-solubilized extracts (10, 25, and 50 μg/ml of medium) of S. horneri. The bone-resorbing factors parathyroid hormone (1-34) (PTH; 10^<-7> M) and prostaglandin E_2 (PGE_2; 10^<-5> M) caused a significant decrease in the diaphyseal and metaphyseal calcium content. The decrease in bone calcium content induced by PTH or PGE_2 was completely inhibited by water-solubilized extracts (10, 25, and 50 μg/ml) of S. horneri. In addition, these extracts (25 and 50 μg/ml) completely prevented the PTH (10^<-7> M)- or PGE_2 (10^<-5> M)-induced increase in medium glucose consumption and lactic acid production by bone tissues. Moreover, S. horneri extracts (10-50 μg/ml) blocked PTH (10^<-7> M)- increased acid phosphatase activity in the diaphyseal and metaphyseal tissues. These findings indicate that water-solubilized extracts of S. horneri have a direct inhibitory effect on bone resorption in tissue culture in vitro.
公益社団法人日本薬学会の論文
2002-04-01