Effect of Polyphenols on Calcium Content and Alkaline Phosphatase Activity in Rat Femoral Tissues in Vitro

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The effect of various polyphenols on calcium content and alkaline phosphatase activity in the femoral-diaphyseal and -metaphyseal tissues of young rats in vitro was investigated. Bone tissues were cultured for 24 h in serum-free Dulbecco's modified Eagle's medium containing either vehicle or various polyphenols(10^<-7>-10^<-4> M). The presence of genistein(10^<-6> -10^<-4> M)caused a significant increase in calcium content and alkaline phosphatase activity in the femoral-diaphyseal and -metaphyseal tissues. Resveratrol(10^<-4> M)decreased metaphyseal calcium content significantly, and it(10^<-6>-10^<-4>M)had a significant inhibitory effect on diaphyseal enzyme activity. Epigallocatechin gallate(EGCg; 10^<-4> M)significantly inhibited alkaline phosphatase activity in the diaphyseal and metaphyseal tissues. EGCg(10^<-7>-10^<-4> M)had no effect on bone calcium content. Meanwhile, glycitein, quercetin, or catechin in the range or 10^<-7> to 10^<-4> M did not have an effect on calcium content and alkaline phosphatase activity in the femoral-diaphyseal and -metaphyseal tissues. The present study suggests that a phytoestrogen genistein has a unique anabolic effect on bone ealcification in vitro.
2001-12-01