Effects of Copper on Bone Component in the Femoral Tissues of Rats : Anabolic Effect of Zinc Is Weakened by Copper(Miscellaneous)
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概要
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The effects of copper on biochemical components in the femoral-diaphyseal (cortical bone) and -metaphyseal (trabecullar bone) tissues of rats in vivo and in vitro were investigated. Rats were orally administered copper sulfate (50, 100, or 200μg Cu/100g body weight) once daily for 7d. Calcium content in the diaphyseal and metaphyseal tissues was significantly decreased with the administration of copper (200μg/100g), while alkaline phosphatase activity in these tissues was not significantly changed by copper administration. The diaphyseal DNA content was significantly decreased with the administration of copper (50, 100, or 200μg/100g). Moreover, the femoral-diaphyseal and -metaphyseal tissues were cultured for 48h in serum-free medium containing either vehicle or copper (10^<-7>-10^<-4>M). Culture with copper (10^<-7>-10^<-4>M) caused a significant decrease in alkaline phosphatase activity in the diaphyseal and metaphyseal tissues, while calcium and DNA contents in these tissues were not significantly changed. Culture with parathyroid hormone [PTH (1-34); 10^<-7>M], a bone-resorbing factor, caused a significant decrease in calcium content in the diaphyseal and metaphyseal tissues. This decrease was completely inhibited in the presence of copper (10^<-6> or 10^<-5>M). Culture with zinc sulfate (10^<-4>M) caused a significant increase in calcium content and alkaline phosphatase activity in the diaphyseal and metaphyseal tissues. The effects of zinc (10^<-4>M) in increasing femoral calcium content and alkaline phosphatase activity were not seen in the presence of cycloheximide (10^<-6>M), an inhibitor of protein synthesis, suggesting that the effects of zinc are involved in newly synthesized protein components. The effects of zinc in increasing calcium content and alkaline phosphatase activity in the diaphyseal and metaphyseal tissues were significantly weakened in the presence of copper (10^<-4>M). The inhibitory effects of copper were further enhanced in the presence of cycloheximide. This study demonstrates that supplementation with copper in adequate copper nutrition does not have anabolic effects on bone components in vivo and in vitro and that copper weakens the anabolic effects of zinc in vitro.
- 公益社団法人日本薬学会の論文
- 2005-12-01
著者
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Yamaguchi Masayoshi
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Lai Ying
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Lai Ying
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Yamaguchi Masayoshi
Laboratories Of Metabolism And Endocrinology School Of Food And Nutritional Sciences The University
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Yamaguchi Masayoshi
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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