Anabolic Effect of Wasabi Leafstalk (Wasabia japonica MATSUM.) Extract on Bone Components in the Femoral-Diaphyseal and -Metaphyseal Tissues of Aged Female Rats in Vitro and in Vivo
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概要
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The effect of wasabi leafstalk (Wasabia japonica MATTUM.) extract on hone components in the femoral-diaphyseal and -metaphyseal tissues of aged female rats in vitro and in vivo was investigated. Femoral-diaphyseal and -metaphyseal tissues were cultured for 48 hr in Dulhecco's modified Eagle's medium (serum free) containing either vehicle or wasabi leafstalk extract (10, 25 or 50 μg/ml of medium). The presence of wasabi leafstalk extract (50 μg/ml) caused a significant increase in calcium content, alkaline phosphatase activity and deoxyribonucleic acid (DNA) content in the diaphyseal and metaphyseal tissues in vitro. However, the effect of wasabi leafstalk extract (50 μg/ml) in increasing hone components was completely abolished in the presence of cycloheximide (10^6 M), an inhibitor of protein synthesis. Moreover, rats were orally administered wasabi leafstalk extract (10 or 20 mg/100 g body weight) once daily for 7 days. The calcium content, alkaline phosphatase activity and DNA content in the femoral-diaphyseal and -metaphyseal tissues of aged rats was significantly increased by the administration of wasabi leafstalk extract (10 or 20 mg/100 g) for 7 days in vivo. Meanwhile, body weight, scrum calcium and inorganic phosphorus concentrations of female aged rats were not significantly altered by the administration of wasabi leafstalk extract (10 or 20 mg/100 g) for 7 days. The present study demonstrates that wasabi leafstalk extract has an anabolic effect on bone components in vitro and in vivo. The intake of wasabi leafstalk extract may have a preventive effect on bone loss with increasing age.
- 公益社団法人日本薬学会の論文
- 2003-04-01
著者
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YAMAGUCHI Masayoshi
Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, Unive
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MA Zhong
Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, Unive
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Ma Zhong
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Yamaguchi Masayoshi
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Suzuki Toshihiro
Functional Food Project Team, Shizuoka Industrial Research Institute of Shizuoka Prefecture
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Ma Zhong
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Suzuki Toshihiro
Functional Food Project Team Shizuoka Industrial Research Institute Of Shizuoka Prefecture
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Yamaguchi Masayoshi
Laboratories Of Metabolism And Endocrinology School Of Food And Nutritional Sciences The University
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Yamaguchi Masayoshi
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Ma Zhong
Laboratory of Endocrinology and Molecular Metabolism,Graduate School of Nutritional Sciences,University of Shizuoka
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Suzuki Toshihiro
Functional Food Project Team,Shizuoka Industrial Research Institute of Shizuoka Prefecture
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