Stimulatory Effect of Wasabi Leafstalk Extract (Wasabia japonica MATSUM.) on Bone Calcification : Interaction with Bone Anabolic Factors in Mouse Calvaria Tissue in Vitro

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The stimulatory effect of wasabi leafstalk extract (Wasabia japonica MATSUM.) on bone calcification was compared with the anabolic effect of insulin, insulin-like growth factor I (IGF-I), 17β-estradiol, and genistein. Mouse calvaria tissues were cultured for 48 h in a serum-free Dulbecco’s modified Eagle’s medium (high glucose, 4.5%). The presence of wasabi leafstalk extract (25 μg/ml) caused significant increases in bone calcium content. The combination of 10−10 M 17β-estradiol and 15 μg/ml wasabi leafstalk extract had an additive effect on bone calcium content, while the increasing effect of phyotoestrogen genistein (10−9 or 10−7 M) on this content was not significantly greater in the presence of the same amount of the extract. Insulin (10−8 or 10−7 M) had a significant effect on bone calcium content, and the combination of both 10−8 or 10−7 M insulin with 15 μg/ml wasabi leafstalk extract had an additive effect. Such an effect was not seen in the case of IGF-I (10−8 M), however which increased bone calcium content. The present study demonstrates that wasabi leafstalk extract has an enhancing effect on the anabolic action of 17β-estradiol or insulin, which regulates bone formation and calcification in vitro.
社団法人　日本食品科学工学会の論文
2003-02-01