Effect of Carotenoid on Calcium Content and Alkaline Phosphatase Activity in Rat Femoral Tissues in Vitro: The Unique Anabolic Effect of β-Cryptoxanthin(Pharmacology)

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The effects of various carotenoids and rutin on calcium content and alkaline phosphatase activity in the femoral-diaphyseal and femoral-metaphyseal tissues of young rats in vitro were investigated. Bone tissues were cultured for 48 h in serum-free Dulbecco's modified Eagle's medium containing either vehicle or a compound (10^<-8>-10^<-6>M). The presence of β-cryptoxanthin (10^<-7>or 10^<-6>M) caused a significant increase in calcium content and alkaline phosphatase activity in the femoral-diaphyseal and femoral-metaphyseal tissues. Also, the lowest concentration (10^<-8>M) of β-cryptoxanthin used caused a significant increase in diaphyseal and metaphyseal calcium content. Lutein (10^<-8>-10^<-6>M) had no effect on diaphyseal and metaphyseal calcium contents and diaphyseal alkaline phosphatase activity, while 10^<-7> and 10^<-6>M lutein significantly decreased metaphyseal alkaline phosphatase activity. Lycopene (10^<-8>-10^<-6>M) or rutin (10^<-8>-10^<-6>M) did not have a significant effect on bone calcium content and alkaline phosphatase activity. The present study suggests that the carotenoid β-cryptoxanthin has a unique anabolic effect on bone calcification in vitro.
公益社団法人日本薬学会の論文
2003-08-01