Characterization of Active Component in Marine Alga Sargassum horneri Extract in Stimulating Bone Calcification in Vitro
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概要
- 論文の詳細を見る
The marine alga Sargassum horneri (S. horneri) has an anabolic effect on bone metabolism (J. Health Sci., 47, 533-538, 2001). The effect of the fractionated extracts obtained from S. horneri on bone calcium content and osteoclast-like cell formation in vitro was investigated. S. horneri was gathered from various coasts (Shimoda, Iwate, or China). Rat femoral-diaphyseal and -metaphyseal tissues were cultured for 24 hr in a medium containing either vehicle or a water-solubilized extract (25 ,μg/ml of medium) obtained from S. horneri. Diaphyseal and metaphyseal calcium contents were significantly increased in the presence of S. horneri extract from Shimoda, Iwate, or China. The active component of S. horneri extract in increasing calcium content in diaphyseal tissues was seen in the nearby fraction molecular weight (MW) of 1000. This effect completely disappeared upon heat treatment with 80℃ for 30 min. Mouse marrow cells were cultured for 7 days in a medium containing 1,25-dihydroxyvitamin D_3[1,25(OH)_2D_3](10^<-7> M) in the presence or absence of 5. horneri extract (1.0 μg/ml of medium). The 1,25(OH)_2D_3 induced increase in osteoclast-like cell formation was markedly suppressed in the presence of the crude extract or a fraction more than MW 50000 of S. horneri extracts obtained from Iwate or China. This effect was also seen by heat treatment. The present study suggests that the active components of S. horneri extract in stimulating bone formation or suppressing osteoclastic bone resorption are different.
- 公益社団法人日本薬学会の論文
- 2004-12-01
著者
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YAMAGUCHI Masayoshi
Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, Unive
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IGARASHI Aki
Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, Unive
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Uchiyama Satoshi
Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, Unive
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Yamaguchi Masayoshi
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Igarashi A
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Igarashi Aki
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Yamaguchi M
Univ. Shizuoka Sizuoka Jpn
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Hashizume Masayuki
Research & Development Department, Maruhachi Muramatsu, Inc.
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Hokari Yoshinori
Research & Development Department, Maruhachi Muramatsu, Inc.
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Nakagawa Takeo
Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, Unive
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Hokari Yoshinori
Functional Food Material Development Laboratory, Maruhachi Muramatsu, Inc.
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Hokari Yoshinori
Functional Food Material Development Laboratory Maruhachi Muramatsu Inc.
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Uchiyama Satoshi
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Hashizume Masayuki
Functional Food Material Development Laboratory Maruhachi Muramatsu Inc.
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Nakagawa Takeo
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Uchiyama Satoshi
Laboratory Of Biology Faculty Of Education And Human Sciences Niigata University
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Yamaguchi Masayoshi
Laboratory Of Endocrinology And Molecular Metabolism Graduate School Of Nutritional Sciences Univers
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Yamaguchi Masayoshi
Laboratories Of Metabolism And Endocrinology School Of Food And Nutritional Sciences The University
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