Purification and Characterization of Mouse Mevalonate Pyrophosphate Decarboxylase
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概要
- 論文の詳細を見る
Mevalonate pyrophosphate decarboxylase (MPD) in mouse liver was purified by affinity chromatography. The purified enzyme was a homodimer of 46-kDa subunits and had an isoelectric point of 5.0. Kinetic analysis revealed an apparent K_m value of 10μM for mevalonate pyrophosphate. The enzyme required ATP as a phosphate acceptor and Mg as a divalent cation, which could be substituted with Mn or Co. Its optimum pH was 4.0-7.0. A comparison with MPD from various other sources revealed the mouse MPD to have essentially the same properties as rat MPD, expect for the optimum pH range. An excess of rabbit anti-rat MPD antibody deleted approximately 80% of the MPD activity in the crude extract of mouse liver. These results suggested that the homodimer of 46-kDa subunits represents the major active form of MPD in mice.
- 公益社団法人日本薬学会の論文
- 2002-03-01
著者
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Yamori Y
Institute For World Health Development Mukogawa Women's University
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Yamori Y
Who Centre For Primary Prevention Of Cardiovascular Diseases Kyoto Jpn
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Yamori Yukio
福山大学 衛生化学
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Yamori Yukio
三楽病院
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MICHIHARA Akihiro
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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AKASAKI Kenji
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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TSUJI Hiroshi
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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MIURA AYAKO
WHO Collaborating Center for Research on Primary Prevention of Cardiovascular Diseases
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Miura Ayako
京都府立医科大学 法医学
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Yamori Y
Departments Of Pathology Shimane Medical University
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Yamori Yukio
Who Collaborating Center
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Yamori Yukio
Japan Stroke Prevention Center And Department Of Pathology Shimane Medical University
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Yamori Yukio
Life Science Environmental Conservation And Development
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Akasaki K
Fac. Of Pharmacy And Pharmaceutical Sciences Fukuyama Univ.
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Akasaki Kenji
Faculty Of Pharmacy & Pharmaceutical Sciences Of Fukuyama University
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Tsuji H
Faculty Of Pharmacy And Pharmaceutical Sciences Fukuyama University
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Yamori Yukio
Departments Of Pathology Shimane Medical University
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Yamori Yukio
Graduate School Of Human And Environmental Studies Kyoto University
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Tsuji Hiroshi
Faculty Of Pharmacy And Pharmaceutical Sciences Fukuyama University
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Akasaki K
Faculty Of Pharmacy And Pharmaceutical Sciences Fukuyama University
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Yamori Yuki
Departments Of Pediatrics And Pathology Shimane Medical University
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Miura Ayako
Department Of Environmental Preservation And Development
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Tsuji Hiroshi
Faculty Of Pharmacy & Pharmaceutical Sciences Of Fukuyama University
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Yamori Yukio
Japan Stroke Prevention Center
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