Distribution of a Major Lysosomal Membrane Glycoprotein, LGP85/LIMP II, in Rat Tissues
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概要
- 論文の詳細を見る
We previously identified and characterized a major lysosomal membrane glycoprotein, termed LGP85 (identical to LIMP II), in rat liver lysosomes. This study describes the distribution of the mRNA and protein of LGP85 in rat tissues. LGP85 protein and mRNA were detectable in all tissues when analyzed by Western and Northern blotting. The 4.2- and 2.2-kb transcripts of LGP85 were detected in all tissues. Liver and lung have the highest and lowest levels of LGP85 mRNA, respectively. A single protein band with an apparent molecular weight (M_r) of 〜85000 was detected in each tissue. The specific protein content of LGP85 in spleen was markedly higher than in other tissues. LGP85 protein is distributed in the tissues independently of LGP85 mRNA. Furthermore, there was a less significant relationship between LGP85 protein and another lysosomal membrane glycoprotein, lamp-1,in the tissue distribution (a regression coefficient of 0.086), which suggests that LGP85 may function in vivo independently of lamp-1.
- 公益社団法人日本薬学会の論文
- 2000-04-01
著者
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AKASAKI Kenji
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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TSUJI Hiroshi
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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Akasaki K
Fac. Of Pharmacy And Pharmaceutical Sciences Fukuyama Univ.
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Akasaki Kenji
Faculty Of Pharmacy & Pharmaceutical Sciences Of Fukuyama University
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Tsuji Hiroshi
Faculty Of Pharmacy And Pharmaceutical Sciences Fukuyama University
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Akasaki K
Faculty Of Pharmacy And Pharmaceutical Sciences Fukuyama University
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TABUCHI Norihiko
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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Tsuji H
Systems Development Laboratory Hitachi Ltd.
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Tabuchi N
Fukuyama Univ. Hiroshima Jpn
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Tsuji Hiroshi
Faculty Of Pharmacy & Pharmaceutical Sciences Of Fukuyama University
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