The Antioxidative and Antilipidemic Effects of Different Molecular Weight Chitosans in Metabolic Syndrome Model Rats
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概要
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The effect of high and low molecular weight chitosans (HMC; 1000 kDa, LMC; 30 kDa) on oxidative stress and hypercholesterolemia was investigated using male 6-week-old Wistar Kyoto rats as a normal model (Normal-rats) and spontaneously hypertensive rat/ND mcr-cp (SHP/ND) as a metabolic syndrome model (MS-rats), respectively. In Normal-rats, the ingestion of both chitosans over a 4 week period resulted in a significant decrease in total body weight (BW), glucose (Gl), triglyceride (TG), low density lipoprotein (LDL) and serum creatinine (Cre) levels. The ingestion of both chitosans also resulted in a lowered ratio of oxidized to reduced albumin and an increase in total plasma antioxidant activity. In addition to similar results in Normal-rats, the ingestion of only HMC over a 4 week period resulted in a significant decrease in total cholesterol levels in MS-rats. Further, the ingestion of LMC resulted in a significantly higher antioxidant activity than was observed for HMC in both rat models. In in vitro studies, LMC caused a significantly higher reduction in the levels of two stable radicals, compared to HMC, and the effect was both dose- and time-dependent. The findings also show that LDL showed strong binding in the case of HMC. These results suggest that LMC has a high antioxidant activity as well as antilipidemic effects, while HMC results in a significant reduction in the levels of pro-oxidants such as LDL in the gastrointestinal tract, thereby inhibiting the subsequent development of oxidative stress in the systemic circulation in metabolic model rats.
著者
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MICHIHARA Akihiro
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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AKASAKI Kenji
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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Miura Ayako
京都府立医科大学 法医学
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Nishio Hiroaki
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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Akasaki Kenji
Faculty Of Pharmacy & Pharmaceutical Sciences Of Fukuyama University
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Nishio Hiroaki
Faculty Of Pharmacy And Pharmaceutical Sciences Fukuyama University
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Anraku Makoto
Fac. Of Pharmacy And Pharmaceutical Sciences Fukuyama Univ.
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Anraku Makoto
Faculty Of Pharmaceutical Sciences Kumamoto University
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Tomida Hisao
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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OTAGIRI Masaki
Graduate School of Pharmaceutical Sciences, Kumamoto University
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Otagiri Masaki
Graduate School Of Pharmaceutical Sciences Kumamoto University
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Maruyama Toru
Graduate School Of Pharmaceutical Sciences Kumamoto Univ.
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Yasufuku Taira
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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Tsuchiya Daiju
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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Maezaki Yuji
Nippon Kayaku Food Techno Co., Ltd.
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Kondo Yuko
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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Maruyama Toru
Graduate School of Pharmaceutical Sciences, Kumamoto University
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