Mevalonate Pyrophosphate Decarboxylase is Predominantly Located in the Cytosol of Rat Hepatocytes

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概要

• 論文の詳細を見る

• Mevalonate pyrophosphate decarboxylase (MPD) is found in the 100000×g supernatant fraction of cells or tissues and is considered to be a cytosolic protein. Recently, other groups reported that MPD is mostly located in the peroxisomes. In this study, we used two different methods to determine whether MPD is predominantly located in the peroxisomes or the cytosol of rat hepatocytes. 1)In permeabilized rat hepatocytes or normal rat kidney cells treated with digitonin, which lack cytosolic enzyme, MPD was mainly present in the medium. 2)Double immunofluorescent labeling of cells with both anti-MPD antibody and anti-hexokinase antibody yielded an immunofluorescent pattern for both enzymes typical of the cytosolic protein. These results indicate that MPD is predominantly located in the cytosol of rat hepatocytes.

• 社団法人日本薬学会の論文
• 2001-11-01

著者

• Yamori Y

  Institute For World Health Development Mukogawa Women's University

• Yamori Y

  Who Centre For Primary Prevention Of Cardiovascular Diseases Kyoto Jpn

• Yamori Yukio

  福山大学 衛生化学

• Yamori Yukio

  三楽病院

• MICHIHARA Akihiro

  Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University

• SAWAMURA Makoto

  Takeda Hospital

• AKASAKI Kenji

  Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University

• TSUJI Hiroshi

  Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University

• MIURA AYAKO

  WHO Collaborating Center for Research on Primary Prevention of Cardiovascular Diseases

• YAMORI Yukio

  Graduate School of Human and Environmental Studies, Kyoto University

• Sawamura M

  Takeda Hospital

• Sagara Miki

  国立国際医療センター研究所

• Sagara Miki

  Research Institute For Production Development

• Miura Ayako

  京都府立医科大学 法医学

• Yamori Y

  Departments Of Pathology Shimane Medical University

• Yamori Yukio

  Life Science Environmental Conservation And Development

• Akasaki K

  Fac. Of Pharmacy And Pharmaceutical Sciences Fukuyama Univ.

• Akasaki Kenji

  Faculty Of Pharmacy & Pharmaceutical Sciences Of Fukuyama University

• Tsuji H

  Faculty Of Pharmacy And Pharmaceutical Sciences Fukuyama University

• Yamori Yukio

  Departments Of Pathology Shimane Medical University

• Yamori Yukio

  Graduate School Of Human And Environmental Studies Kyoto University

• Tsuji Hiroshi

  Faculty Of Pharmacy And Pharmaceutical Sciences Fukuyama University

• Akasaki K

  Faculty Of Pharmacy And Pharmaceutical Sciences Fukuyama University

• Yamori Yuki

  Departments Of Pediatrics And Pathology Shimane Medical University

• Miura Ayako

  Department Of Environmental Preservation And Development

• Tsuji Hiroshi

  Faculty Of Pharmacy & Pharmaceutical Sciences Of Fukuyama University

• Yamori Yukio

  Graduate School of Human and Environmental Studies

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