EFFECTS OF E-1020, A NEW CYCLIC AMP-SPECIFIC PHOSPHODIESTERASE INHIBITOR, ON CYCLIC AMP AND CYTOSOLIC FREE CALCIUIM OF CULTURED VASCULAR SMOOTH MUSCLE CELLS : The 53th Annual Scientific Session of the Japanese Circulation Society

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概要

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• The purpose of this study is to clarify the pharmacological effects of E-1020, a new cAMP-specific phosphodiesterase (PDE) inhibitor, on CAMP or cytosolic free calcium (Ca) of cultured vascular smooth muscle cells (VSMC). VSMC were separated from the thoracic aorta of 2- to 3-month-old Wistar Kyoto rats (WKY). CAMP was measured by using the ^<125>I-cAMP radioimmunoasay kit. The cytosolic free Ca of VSMC was measured by using the fluorescence Ca indicator. Fura-2 / acetoxymethyl ester (Fura-2/AM). As a result. E-1020 increased the CAMP of VSMC in a dose-dependent manner, and about two fold increase in CAMP was observed by 10-4M E-1020. E-1020 decreased the cytosolic free concentration of Ca in a dose-dependent manner, and a significant decrease in cytosolic free Ca was observed by >10^<-6>M E-1020. These effects of E-1020 on CAMP and cytosolic free Ca were enhanced in the presence of low concentration (10;^<>;8M) of DL-noradrenaline. That is, a more prominent decrease in cytosolic free Ca than that of E-1020 alone was observed. Our results show that E-1020 decreases cytosolic free Ca of VSMC in parallel with increase in cAMP, and thereby suggest a potential vasodilating activity in vivo.

• 社団法人日本循環器学会の論文
• 1990-06-20

著者

• Morioka Shigefumi

  Department of Cardiology, Kobe City Medical Center General Hospital

• Honda Masaaki

  Department of Computer Engineering, Waseda University

• Yamori Y

  Institute For World Health Development Mukogawa Women's University

• Yamori Y

  Who Centre For Primary Prevention Of Cardiovascular Diseases Kyoto Jpn

• Yamori Yukio

  福山大学 衛生化学

• Yamori Yukio

  Department Of Pathology Faculty Of Medicine Kyoto University

• HONDA MASAAKI

  The 4th Department of Internal Medicine, Shimane Medical University

• ISHIKAWA SHIGENORI

  The 4th Department of Internal Medicine, Shimane Medical University

• OHOKA MICHIKUNI

  Department of Pathology Shimane Medical University

• ISHIKAWA SHIGENORI

  Department of Internal Medicine

• NAKAYAMA KAZUAKI

  Department of Internal Medicine

• MORIYAMA KATSUTOSHI

  Department of Internal Medicine

• Yamori Yukio

  Who Collaborating Center

• Honda Masaaki

  The 4th Department Of Internal Medicine Shimane Medical University

• Morioka Shigefumi

  Fourth Department Of Internal Medicine Shimane Medical University

• Moriyama Katsutoshi

  The Fourth Department Of Internal Medicine Shimane University

• Ishikawa Shigenori

  The 4th Department Of Internal Medicine Shimane Medical University

• Yamori Yukio

  Departments Of Pathology Shimane Medical University

• Morioka Shigefumi

  Department Of Cardiology Kobe City Medical Center General Hospital

• Nakayama Kazuaki

  Department Of Cardiovascular Medicine Okayama University Medical School

• Honda Masaaki

  Department Of Computer Engineering Waseda University

• Yamori Yukio

  Department of Life Science, Graduate School of Human and Environmental, Kyoto University

• Honda Masaaki

  Department of Chemistry, Faculty of Science, Kumamoto University

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