Difference in Subcellular Distribution of Mevalonate Pyrophosphate Decarboxylase Occurs by Cell Type
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概要
- 論文の詳細を見る
Recently, it has been questioned whether mevalonate pyrophosphate decarboxylase (MPD) is predominantly located in the peroxisomes or cytosol. We previously reported that MPD was predominantly present in the cytosol of rat hepatocytes, normal rat kidney cells, or mouse melanoma cells. In the present study, we examined whether MPD was predominantly present in the cytosol of HepG2 (human hepatoma) cells and Cos7 (monkey kidney) cells using digitonin permeabilization. In HepG2 cells permeabilized with digitonin, 90% and 10% of MPD existed in the cytosol and membrane/organelle (M/O) fraction, respectively, while in Cos7 cells permeabilized with digitonin, 20% and 80% of MPD existed in the cytosol and M/O fraction, respectively. These data suggest that the difference in subcellular distribution of MPD is due to the cell type.
- 公益社団法人日本薬学会の論文
- 2008-12-01
著者
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MICHIHARA Akihiro
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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AKASAKI Kenji
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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TSUJI Hiroshi
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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MIURA AYAKO
WHO Collaborating Center for Research on Primary Prevention of Cardiovascular Diseases
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Miura Ayako
京都府立医科大学 法医学
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Akasaki K
Fac. Of Pharmacy And Pharmaceutical Sciences Fukuyama Univ.
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Akasaki Kenji
Faculty Of Pharmacy & Pharmaceutical Sciences Of Fukuyama University
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Tsuji H
Faculty Of Pharmacy And Pharmaceutical Sciences Fukuyama University
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Tsuji Hiroshi
Faculty Of Pharmacy And Pharmaceutical Sciences Fukuyama University
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Akasaki K
Faculty Of Pharmacy And Pharmaceutical Sciences Fukuyama University
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Morita Sachiyo
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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Nakayama Masahiro
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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Kubo Yayoi
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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Miura Ayako
Department Of Environmental Preservation And Development
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Kubo Yayoi
Faculty Of Pharmacy And Pharmaceutical Sciences Fukuyama University
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Nakayama Masahiro
Faculty Of Pharmacy And Pharmaceutical Sciences Fukuyama University
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Tsuji Hiroshi
Faculty Of Pharmacy & Pharmaceutical Sciences Of Fukuyama University
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Nakamura Masahiro
Faculty Of Pharmaceutical Sciences Kyushu University
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