Hydrolytic Cleavage of Pyroglutamyl-peptide Bond. III. A Highly Selective Cleavage in 70% methanesulfonic Acid
スポンサーリンク
概要
- 論文の詳細を見る
A method for highly selective cleavage of pGlu-peptide linkages in 70% methanesulfonic acid (MSA) is described. When pGlu-Ala-Phe-OH, pGlu-His-Pro-OH and dog neuromedin U-8 (d-NMU-8) (1-7)-OH (pGlu-Phe-Leu-Phe-Arg-Pro-Arg-OH) were hydrolyzed in 70% MSA at 60℃ for 3 h or at 25℃ for 3 d, the pGlu-peptide linkage was predominantly cleaved to give H-Ala-Phe-OH, H-His-Pro-OH and H-Phe-Leu-Phe-Arg-Pro-Arg-OH, in high yields. The results indicated that pGlu-peptide linkages are highly susceptible to 70% MSA, whereas the amide bond of the pyrrolidone moiety of the pGlu residue and other internal peptide bonds are extremely resistant.
- 公益社団法人日本薬学会の論文
- 1996-11-15
著者
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OKIMURA Keiko
Faculty of Pharmaceutical Sciences, Hokuriku University
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SAKURA Naoki
Faculty of Pharmaceutical Sciences, Hokuriku University
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橋本 忠
北陸大・薬
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Ohnishi Kuniharu
Faculty Of Pharmaceutical Sciences Hokuriku University
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Ohnishi Kuniharu
北陸大学 薬学部
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Ohki K
Faculty Of Pharmaceutical Sciences Hokuriku University
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Ohki Kazuhiro
Faculty Of Pharmaceutical Sciences Hokuriku University
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Sakura N
Hokuriku Univ. Kanazawa Jpn
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Sakura Naoki
北陸大学 薬学部
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Sakura Naoki
Faculty Of Pharmaceutical Sciences Hokuriku University
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橋本 忠
北陸大学薬学部
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Hashimoto Tadashi
Faculty Of Pharmaceutical Sciences Hokuriku University
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Hashimoto T
Hokuriku Univ. Kanazawa Jpn
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Hashimoto Toshihiro
Fac. Of Pharmaceutical Sciences Tokushima Bunri Univ.
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