HPLC Analysis of Fatty Acyl-Glycine in the Aqueous Methanesulfonic Acid Hydrolysates of N-Terminally Fatty Acylated Peptides(Analytical Biochemistry)

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概要

• 論文の詳細を見る

• Acylation with long-chain fatty acids is a common modification at the N-terminal glycine residues of natural proteins. In this work, we performed HPLC analysis of myristoylglycine (Myr-Gly-OH), palmitoylglycine (Pal-Gly-OH) or lauroylglycine (Lau-Gly-OH), which were produced in the hydrolysates of synthetic Myr-Gly-, Pal-Gly-, or Lau-Gly-peptides, respectively, by means of a mild acid hydrolysis in methanesulfonic acid: dioxane: water (2:1:1) at 60℃ for 12 h. Myr-Gly-OH, Pal-Gly-OH and Lau-Gly-OH were quite stable under hydrolysis conditions. These fatty acyl-Gly-OH were conveniently detectable at a 20nmol level by direct reversed-phase HPLC. Thus, mild acid hydrolysis, followed by HPLC analysis of the hydrolysate, provides a simple method of identification of the N-terminal structure of fatty acyl-Gly-peptides.

• 社団法人日本薬学会の論文
• 2003-08-01

著者

• OHKI Kazuhiro

  Faculty of Pharmaceutical Sciences, Hokuriku University

• OKIMURA Keiko

  Faculty of Pharmaceutical Sciences, Hokuriku University

• SAKURA Naoki

  Faculty of Pharmaceutical Sciences, Hokuriku University

• Ohnishi Kuniharu

  Faculty Of Pharmaceutical Sciences Hokuriku University

• Ohnishi Kuniharu

  北陸大学 薬学部

• Ohki K

  Faculty Of Pharmaceutical Sciences Hokuriku University

• Ohki Kazuhiro

  北陸大学 薬学部

• Ohki Kazuhiro

  Faculty Of Pharmaceutical Sciences Hokuriku University

• Okimura K

  Faculty Of Pharmaceutical Sciences Hokuriku University

• Okimura Keiko

  北陸大学 薬学部

• Okimura Keiko

  Faculty Of Pharmaceutical Sciences Hokuriku University

• 佐倉 直樹

  北陸大・薬

• Sakura N

  Hokuriku Univ. Kanazawa Jpn

• Sakura Naoki

  北陸大学 薬 生物活性

• Sakura Naoki

  北陸大学 薬学部

• Sakura Naoki

  Faculty Of Pharmaceutical Sciences Hokuriku University

• Nagai Satoo

  Faculty Of Pharmaceutical Sciences Hokuriku University

• NAGAI Sotoo

  Faculty of Pharmaceutical Sciences, Hokuriku University

• Nagai S

  Faculty Of Pharmaceutical Sciences Hokuriku University

• Nagai Sotoo

  Faculty Of Pharmaceutical Sciences Hokuriku University

• Nagai Sotoo

  北陸大学 薬学部

• Natori S

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

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