Regioselective Hydrolytic Cleavage of N-Terminal Myristoyl-peptide

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概要

• 論文の詳細を見る

• To develop a simple chemical deblocking method for Myr-Gly-peptide, we investigated the susceptibility and stability of the internal peptide linkages of model peptides, Myr-Gly-X-Phe-OH (X=Gly, Ala, Val, Lys, His, Arg, Ile, Glu, Gln, Asp, Asn), to HCl, H_2SO_4 and methanesulfonic acid (MSA) at 25 and 60℃, and analyzed the hydrolysates by HPLC. The results indicated that acid hydrolysis in concentrated acids was superior for selective cleavage of the Gly-X linkage. Here, we describe a simple method for regioselective cleavage of the Gly-X bond of Myr-Gly-X-Phe-OH by acids and determination of the decomposition products by RP-HPLC.

• 社団法人日本薬学会の論文
• 1999-09-15

著者

• OHKI Kazuhiro

  Faculty of Pharmaceutical Sciences, Hokuriku University

• OKIMURA Keiko

  Faculty of Pharmaceutical Sciences, Hokuriku University

• SAKURA Naoki

  Faculty of Pharmaceutical Sciences, Hokuriku University

• HASHIMOTO Tadashi

  Faculty of Pharmaceutical Sciences, Hokuriku University

• Hashimoto T

  Faculty Of Pharmaceutical Sciences Hokuriku University

• 橋本 忠

  北陸大・薬

• Ohnishi Kuniharu

  Faculty Of Pharmaceutical Sciences Hokuriku University

• Ohnishi Kuniharu

  北陸大学 薬学部

• Ohki K

  Faculty Of Pharmaceutical Sciences Hokuriku University

• Ohki Kazuhiro

  北陸大学 薬学部

• Ohki Kazuhiro

  Faculty Of Pharmaceutical Sciences Hokuriku University

• Okimura K

  Faculty Of Pharmaceutical Sciences Hokuriku University

• Okimura Keiko

  北陸大学 薬学部

• Okimura Keiko

  Faculty Of Pharmaceutical Sciences Hokuriku University

• Sakura N

  Hokuriku Univ. Kanazawa Jpn

• Sakura Naoki

  Faculty Of Pharmaceutical Sciences Hokuriku University

• SUGIMOTO Kaori

  Faculty of Pharmaceutical Sciences, Hokuriku University

• Sugimoto Kaori

  Faculty Of Pharmaceutical Sciences Hokuriku University

• Hashimoto Tadashi

  Faculty Of Pharmaceutical Sciences Hokuriku University

• Sugimoto Kenji

  江崎グリコ生物化学研究所

• Hashimoto T

  Hokuriku Univ. Kanazawa Jpn

• Hashimoto Toshihiro

  Fac. Of Pharmaceutical Sciences Tokushima Bunri Univ.

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