2,3-ヂオキシ安息香酸特異性の新デカルボキラーゼ, 並びにこれがクロカビのサリチル酸代謝に於ける役割

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A strain of Asp. niger capable of decomposing salicylic acid (SA) to form catechol, oxalic acid and CO_2,has been employed. On the basis of previous observations (TERUI and TOKAKU), that catechol does not give rise to the formation of oxalic acid by this organism, 2,3-dihydroxybenzoic acid (2,3-DOBA) has been assumed to be the intermediate in the route of SA metabolism. In fact, 2,3-DOBA was proved by these authors to be an excellent substrate for the formation of both catechol and oxalic acid. Subsequently, a decarboxylase specific for 2,3-DOBA (doba decarboxylase) was extracted from the mycelia of Asp. niger previously adapted to the substrate. The enzyme catalizes simple decarboxylation of 2,3-DOBA to form catechol but exerts practically no action on benzoic, salicylic, p-hydroxybenzoic, protocatechuic, β-resorcylic, gentisic, 2,3,4-trihydroxybenzoic, gallic, p-aminobenzoic and anthranilic acid. It has been established also that 2,3-DOBA is the intermediate of SA metabolism on the basis of adaptive pattern, i.e. the formation of hte decarboxylase by the adaptation of the mycelia either to 2,3-DOBA or to SA.Although enzymological study of doba decarboxylase is now in progress, it has been evidnced that any prosthetic group easily dissociable or coenzyme is absent in this enzyme. Competitive inhibition of the enzyme by SA, relatively low optimum pH (5.2) and high affinity to the substrate (Michaelis constant : 0.0035 M) are the remarkable properties. (cf. Fig. 1〜8 in p. 66〜70).
公益社団法人日本生物工学会の論文
1953-02-15

2,3-ヂオキシ安息香酸特異性の新デカルボキラーゼ, 並びにこれがクロカビのサリチル酸代謝に於ける役割

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