ピルビン酸醗酵に關する研究 : (第2報) Mucor mandshuricus Saito によるビタミンB_1定量の可能性に就て

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The accumulation of pyruvic acid by M. mandshuricus was suppressed when vitamin B_1 had been added to the culture medium. The reason of this phenomenon is considered that the enzyme system which transforms pyruvic acid to the substances of next stage will be reinforced by vitamin B_1.The minimum concentration of B_1 which suppresses perfectly the accumulation of pyruvic acid by M. mandshuricus is 2 γ% at present experimental conditions, but this value may be varied by the nature of preliminary culture medium.Experimentally, following equation exists between vitamin B_1 concentration B (γ/V) and pyruvic acid accumulation P (mg/V : )-2 log_10B=tan P Therefore, the authors pointed out the possibility of biochemical determination for vitamin B_1. (Where V : - Cubic centimeters of culture medium which contains 90 mg of pyruvic acid in the vitamin free control flask).Examination of the conditions in detail for the biochemical determination of B_1 by the accumulation of pyruvic acid, is still remained as a future problem.however, M.mandshuricus is considered to have a superiority over Phycomyces blakesleeanus.
社団法人日本生物工学会の論文
1951-08-15