乳酸菌の酵素に關する研究 : (第4報)葡萄糖とPyruvic Acidの共軛分解

概要

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In the resting state, homofermentative lactic acid bacteria (L. plantarum) decomposes glncose theoretically to lactic acid, whilst bacterofermenter (Leuconostoc mesenteroides) has no action. But if pyruvic acid was substituted for glucose, neither homofermenter nor heterofermenter is able to decompose it. These facts show that there may be fundamental difference between the enzymic systems of the two main types of lactic acid bacteria, althongh both of them are lacking in carboxylase.When mixture of glucose and pyruvic acid was used as substrate, vigorous conpled reaction of almost zero order was observed, and the molecular ratio of glucose to pyruvic acid was 1 : 2. From analytical data the author proposed the following formulas.Homofermenter : C_6H_<12>O_6+2 CH_8COCOOH=2 CH_8CHOHCOOH+2(O_2+CH_8CHOHCOCH_8 Heterofermenter : C_6H_<12>O_6+2 CH_8COCOOH+H_2O=2 CH_8CHOHCOOH+2 CO_2+C_2H_5OH+CH_8COOH It is very interesting to see that the main products ; lactic acid and CO_2 coincide with the two types of bacteria, thus the fundamental difference between them disappears. Therefore the present author concluded the key to differencate homofermentation and heterofermentation may not be carboxylase, (hypothesis of Peerson etc.), but will have to be sought somewhere else and this conpled reaeion will offer some hints to solve this question.
公益社団法人日本生物工学会の論文
1949-05-15