絲状菌類のDiastase組成に關する研究(第2報) : 各麹Diastaseの組成比並に黒麹Amylaseの特異性に就て

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To obtain the numerical constructive ratio of each mold-idastase, we attempted to modify ohlsson's method to be applicable simply to mold-diastase.In cases of Asp. Oryzac, Rh. javanicus and white aspergillus, we succeeded to isolate α-amylase, when diastase solution was treated at pH 7.0,55° for 15 minute. And this partial enzyme could be eliminated by treating the diastase solution at pH 3.6,50° for 15 min. thus obtaining the mixture of β-amylase and maltase.But we could never separate β-amylase and maltase, because their physical properties are quite identical.Though based on some assumptions, we abtained the activity ratio of each mold-diastase as follows.[table]Diastase of Asp. awamori sp. has the special characteristics, that the above mentioned methods of separation of partial enzymes could not be applied, because α-amylase of this mold is very aciduric, and β-amylase is comparatively heat stable. From these specificities it may be inferred, that in this mold-diastase, the enzymic carriers of α- and β- amylase may be exchanged each other.Namely, in ordinary mold : -α-Amylase=Co-enzyme α+Carrier A, β-Amylase=Co-enzyme β+Carrier B. but in Asp. awamori sp. : -α-Amylase=Co-enzyme α+Carrier B, β-Amylase=Co-enzyme β+Carrier A.
公益社団法人日本生物工学会の論文
1949-04-15