運動および死滅ウシ精子の5400G定常磁場配向に及ぼす膜表面荷電物質シアル酸の影響

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The present paper describes the effect of cell surface sialic acid on the orientation of motile and killed sperm of cattle in a 5400-G magnetic field. Cattle sperm collected from six bulls was placed in the medium. The semen suspensions were divided into motile, freeze-killed, and naturally dead sperm samples. An aliquot of the sperm samples were incubated with an added 1.0 mg/ml neuraminidase at 30°C for 1 hour. Neuraminidase-treated and non-treated samples were exposed continuously for 24 hours to a magnetic field of 5400G at 38°C. Neuraminidase treatment enhanced the orientation of the sperm. Naturally dead sperm treated with neuraminidase and exposed to the mgnetic filed had the highest percentage of orientation. These findings suggest that a negatively charged cell surface inhibits sperm from orientating. However, freeze-killed sperm had a lower percentage of orientation than naturally dead and motile sperm.
1998-04-01