5400G定常磁場における除膜および核膨潤化ウシ精子の磁場配向の高低

概要

論文の詳細を見る
This paper describes the orientation of demembranated and nuclear-decondensed cattle sperm treated with 1% glutaraldehyde in a 5400-G static magnetic field. The sperm collected from four bulls was mixed with the same sperm density. One-percent triton X-100 was used to extract the sterm plasma membrane, while 10 mM dithiothreitol and heparin sodium were used to decondense the sperm nucleus. The intact, demembranated, and nucleardecondensed sperm non-treated or treated with glutaraldehyde were exposed continuously for 24 hours to a 5400-G magnetic field at 25℃. The demembranation and treatment with glutaraldehyde enhanced the orientation of the sperm. However, nuclear-decondensed sperm, as well as non-exposed sperm, had a very low perpendicularly oriented percentage, while the flagella showed low parallel orientation. These findings suggest that the magnetization of cattle sperm heads occurs in the chromatin.
1998-06-01