Purification and Some Properties of α-Amylase from Bacillus subtilis X-23 That Glucosylates Phenolic Compounds Such as Hydroquinone

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Six hundred strains of soil microorganisms were screened for the production of hydroquinone glucosylating enzyme (HGE). One of these strains, Bacillus subtilis strain X-23,produced an enzyme that glucosylated hydroquinone in the culture filtrate. The HGE was successively purified by ammonium sulfate fractionation, and Q-Sepharose, Phenyl-Toyopearl, and Superose 12 column chromatographies. The molecular weight was estimated as 65 kDa by SDS-polyacrylamide gel electrophoresis, and 54 kDa by gel filtration. Based on an analysis of the hydrolytic products from soluble starch, HGE was considered to be a kind of α-amylase. The structure of the hydroquinone glucoside produced by HGE was identified as 4-hydroxyphenyl-O-α-D-glucopyranoside by α- and β-glucosidase treatments, and ^1H-NMR and ^<13>C-NMR.
社団法人日本生物工学会の論文
1994-07-25