A Novel Acid Phosphatase from Aspergillus niger KU-8 That Specifically Hydrolyzes C-6 Phosphate Groups of Phosphoryl Oligosaccharides
スポンサーリンク
概要
- 論文の詳細を見る
We had analyzed the detailed structures of the phosphoryl oligosaccharide-1 (PO-1) fraction that was the main component of phosphoryl oligosaccharides (POs) prepared from a potato starch hydrolysate. PO-1 fraction was made up of 3-phosphoryl oligosaccharides and 6-phosphoryl oligosaccharides. Aspergillus niger strain KU-8 produced two types of intracellular acid phosphatase (EC 3.1.3.2, ACPase); ACPase I and II. ACPase II preferentially dephosphorylated 6-phosphoryl oligosaccharides rather than 3-phosphoryl oligosaccharides. The molecular weight of the enzyme was estimated as 66kDa by SDS-polyacrylamide gel electrophoresis and about 260kDa by gel filtration, implying the active form to be a tetramer. The optimum pH and temperature of the enzyme were 2.0-2.5 and 60℃, respectively. ACPase II was stable below 50℃ for 30 min and pH 2.0-10.0 for 60 min. In spite of the strict specificity toward 6-phosphoryl oligosaccharides in the PO-1 fraction, ACPase II was able to hydrolyze Fru-1, 6-di-P, ATP, pyrophosphate, and polyphosphate as well as pNPP and Glc-6-P, a broad substrate specificity.
- 社団法人日本農芸化学会の論文
- 1997-09-23
著者
-
KURIKI Takashi
Biochemical Research Laboratory, Ezaki Glico Co., Ltd.
-
Kuriki T
Health Science Laboratory Ezaki Glico
-
Kometani T
Biochemical Res. Lab. Ezaki Glico Co. Ltd.
-
Kuriki T
Ezaki Glico Co. Ltd. Osaka Jpn
-
KAMASAKA Hiroshi
Biochemical Research Laboratory, Ezaki Glico Co., Ltd.
-
Kometani Takashi
Biochemical Research Laboratory, Ezaki Glico Co., Ltd.
-
Okada Shigetaka
Biochemical Research Laboratory, Ezaki Glico Co., Ltd.
-
TO-O Kenji
Biochemical Research Laboratory, Ezaki Glico Co., Ltd.
-
KUSAKA Kaname
Biochemical Research Laboratory, Ezaki Glico Co., Ltd.
-
To-o Kenji
Biochemical Research Laboratory Ezaki Glico Co. Ltd.
-
To-o Kenji
Health Science Laboratory Ezaki Glico
-
Kuriki Takashi
Biochemical Research Laboratory Ezaki Glico
-
Kuriki Takashi
Biochemical Research Laboratories Ezaki Glico Co. Ltd.
-
Kamasaka Hiroshi
Biochemical Research Laboratory Ezaki Glico Co. Ltd.
-
Kusaka Kaname
Biochemical Research Laboratory Ezaki Glico Co. Ltd.
-
Kuriki T
Biochemical Research Laboratory Ezaki Glico
-
Kamasaka H
Biochemical Research Laboratory Ezaki Glico Co. Ltd.
-
Kometani Takashi
Biochemical Research Laboratory Ezaki Glico Co. Ltd.
-
Okada S
Biochemical Research Laboratory Ezaki Glico Co. Ltd.
-
Okada Shigetaka
Biochemical Research Laboratory Ezaki Glico Co. Ltd.
関連論文
- α-アルブチンの開発 : 工業スケールでの製造および美白化粧品原料への応用
- Experimental study on cross-reactivity of α-arbutin toward p-phenylenediamine and hydroquinone in guinea pigs
- Highly Branched Oligosaccharides Produced by the Transglycosylation Reaction of Neopullulanase
- Neopullulanase Exhibits Distinct Specificity toward Amylose and Amylopectin
- Synthesis of 3,4-Dimethoxyphenyl β-D-Glucopyranoside and Its Related Glycosides by Cultured Plant Cells
- Glucosylation of Capsaicin by Cell Suspension Cultures of Coffea arabica
- Glucosylation of Vanillin by Cultured Plant Cells
- A New Method for Precipitation of Various Glucosides with Cyclodextrin Glucano-transferase
- 馬鈴薯澱粉由来リン酸化オリゴ糖の生産と応用
- Novel Transglucosylating Reaction of Sucrose Phosphorylase to Carboxylic Compounds Such as Benzoic Acid(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)