Neopullulanase Exhibits Distinct Specificity toward Amylose and Amylopectin
スポンサーリンク
概要
- 論文の詳細を見る
Neopullulanase was the key enzyme to open the door for the formulation of the concept of the aamylasefamily. The enzyme catalyzes both hydrolysis and transglycosylation at α-1, 4- and α-1, 6-glucosidic linkages through one active center. We found a unique macromolecule recognition by the enzyme. A mixture of amylose and amylopectin from various sources was used as the substrate. Neopullulanase completely hydrolyzedamylose to maltose and a small amount of glucose, but scarcely hydrolyzed amylopectin. Although the molecular mass of potato amylopectin (approximately 10<SUP>8</SUP> Da) decreased slightly, the degradation of amylopectincompletely halted at the molecular mass of approximately 10<SUP>7</SUP>Da. This difference in action on two macromolecules was also found in cyclomaltodextrinase and maltogenic amylase from Bacillus licheniformis.
- 日本応用糖質科学会の論文
- 2003-07-14
著者
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TO-O Kenji
Health Science Laboratory, Ezaki Glico
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Kuriki T
Health Science Laboratory Ezaki Glico
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Kometani T
Biochemical Res. Lab. Ezaki Glico Co. Ltd.
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Kuriki T
Ezaki Glico Co. Ltd. Osaka Jpn
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Takata Hiroki
Biochemical Research Laboratory Ezaki Glico Co. Ltd.
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To-o Kenji
Health Science Laboratory Ezaki Glico
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Kuriki Takashi
Biochemical Research Laboratory Ezaki Glico
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Kuriki Takashi
Biochemical Research Laboratories Ezaki Glico Co. Ltd.
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Kamasaka Hiroshi
Biochemical Research Laboratory Ezaki Glico Co. Ltd.
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Sugimoto Kazuhisa
Biochemical Research Laboratory Ezaki Glico
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Sugimoto Kazuhisa
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Nishimura Takahisa
Biochemical Research Laboratory Ezaki Glico Co. Ltd.
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Nishimura Takahisa
Research Planning Division Ezaki Glico
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Nishimura T
Central Research Laboratories Kissei Pharmaceutical Co. Ltd.
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Kamasaka H
Biochemical Research Laboratory Ezaki Glico Co. Ltd.
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