Purification and Characterization of Two Lectins from Callus of Helianthus tuberosus

概要

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Two lectins were purified from Helianthus tuberosus callus by maltose affinity chromatography and subsequent preparative electrophoresis. The lectins were designated HTAI and HTAII and their molecular masses were about 34 kDa by gel-filtration chromatography. A single band of 17 kDa and bands of 17 kDa and 18 kDa were detected after SDS-PAGE of HTA I and HTA II, respectively; indicating that HTAI is a homodimer while HTAII is a heterodimer. The amino acid compositions of the two lectins were very similar; they were rich in glycine residues, lacking detectable amounts of methionine, cysteine, and histidine. A hapten-inhibition assay showed that HTA I and HTA II had identical saccharide-binding specificity to the extent tested and belonged to the group of so-called mannose/glucose-binding lectins. They had high affinity forα-linked manno-oligosaccharides. Each HTA completely lost its hemagglutinatinig activity at pH 5.0, as a result of its dissociation to monomers, but it did not lose its ability to bind to oligosaccharides.
社団法人日本農芸化学会の論文
1996-02-23