透析膜を被覆したグラッシーカーボン電極を用いる血清中のγ-グルタミルトランスペプチダーゼ活性の測定

概要

論文の詳細を見る
The activity of γ-glutamyltranspeptidase (γGTP) in serum was determined by measuring the increase in current for a given time due to the oxidation of 5-aminosalicylic acid, as enzymatically released from γ-glutamyl-3-carboxy-4-hydroxyanilide in Tris buffer of pH 8.5 containing glycylglycine, with a dialysis membrane-covered glassy-carbon electrode. The activities determined amperometrically for twenty six serum samples were in excellent agreement with those determined by a colorimetric method using γ-glutamyl-p-nitroanilide as the substrate (with the correlation coefficient of 0.997). The electrode was free of deactivation of electrode surface by the protein adsorption typically encountered when an electrode without a dialysis membrane was employed.
社団法人日本分析化学会の論文
1993-03-05