オリゴ糖デヒドロゲナーゼを固定したビタミンK_3練り込みグラファイトペースト電極を用いる血清中のグルコースの定量及びアミラーゼ活性の測定

概要

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Oligosaccharide dehydrogenase modified graphite paste electrode containing vitamin K_3 produced biocatalytic current due to the oxidation of glucose and oligosaccharide in the potential region where the oxidation of ascorbic acid and uric acid do not occur. The magnitude in current due to the saccharides was inversely proportional to the amount of glucose in saccharide. The glucose concentration in serum determined by measuring the current at -100mV and pH8.0 agree well that by a glucokinase-UV method (r=0.997 n=20) Five min after the addition of maltopentaose to the buffer solution containing serum, the current increased linearly and the magnitude of increase for a given time was proportional to the amount of serum. This increase in current based on the hydrolysis of maltopentaose to the maltose and maltotriose by amylase. So amylase activity can be easily determined by measuring the current increase with time after determination of glucose concentration in serum and the addition of maltopentaose.
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