フルクトシルアミノ酸オキシダーゼ及びプロティナーゼKを用いる血清フルクトサミンのアンペロメトリー

概要

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The concentration of fructosamine (FSA) in serum, reflecting the level of blood glucose during two weeks, was determined by an amperometric measurement of H2O2 generated by fructosyl amino acid oxidase (FAOD) after degradation of the serum protein by proteinase K (PK) using a membrane-covered, peroxidase-entrappedand ferrocene -embedded carbon paste electrode. After 20 min degradation of 1/25 diluted serum by 10 U ml-1 PK, FSA was determined by measuring the current increase after the addition of 20 U ml-1 FAOD at pH 9. The ratio of the determined FSA concentration using the current magnitude of N-fructosyl N-Z-lysine (FZL) as a standard to that using diagonostic FSA calibrator as a standard was 0.8. The correlation coefficient between the FSA concentration determined in this method and that by a diagonostic kit based on the spectrometric measurement was 0.97 for 14 serum samples.
社団法人　日本分析化学会の論文
2002-10-05