コバルトフタロシアニン練り込みカーボンペースト電極のスルフィドリル化合物に対する応答及び酵母エキス中のグルタチオン定量への応用

概要

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By using an unmodified carbon electrode, a sulfhydryl compound can be detected in a potential region more positive than 400 mV vs. SCE, where currents by uric acid, phenols and NADH are also detected. A membrane-covered cobalt phthalocyanine embedded carbon-paste electrode(CoPCP electrode) allows the detection of glutathion(GSH) as low as 2 μM at 200 mV with practically no influence from coexisting substances in a biological sample, except for another sulfhydryl compound and ascorbic acid. The GSH contents in tweleve samples of yeast extract measured by a CoPCP electrode and those by a photometric method using glyoxalase I are in good agreement with the correlation coefficient of 0.96. The current by GSH increased 3.5 times with the addition of 5,5'-dithiobis-(2-nitrobenzoic acid)(DTNB), since the membrane pearmiability of GSH and TNB formed from DTNB by GSH are quite different from each other. Similarly, the current by yeast extract increased 3.5 times with the addition of DTNB. This result confirms that the current by yeast extract was practically due to GSH. The current due to cysteine was reduced by 13% upon the addition of DTNB.
社団法人日本分析化学会の論文
1999-01-05