透析膜を被覆したグラファイトペースト電極を用いる血清尿酸のボルタメトリック定量

概要

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A dialysis membrane-covered graphite paste electrode was prepared and tested as a detecting electrode for uric acid in serum. The current magnitude of uric acid on the linear sweep voltammograms was proportional to the concentration of uric acid from 1 μM to 200 μM. Protein, which fouls the surface of a bare lectrode and worsens the property of the electrode, did not affect the anodic current of uric acid at this electrode. Oxidizable compounds in serum except ascorbic acid did not interfere with the current of uric acid measured at 0.5-0.6 V in the acidic solutions by this electrode. The magnitude of the anodic current for a serum solution measured in pH 3-4 could be corrected for the effect of ascorbic acid to obtain the current due to uric acid. Values of uric acid content in serum measured by this method agreed with those by a photometric uricase-peroxidase procedure.
活水女子大学の論文