Catalase Assay Using a Dialysis Membrane-Covered Platinum Electrode

概要

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A simple method for the measurement of the catalase activity, which uses an ordinary platinum electrode covered with a dialysis membrane is proposed. The anodic current measured at+0.6 V vs. saturated-calomel electrode (SCE) is proportional to the concentration of hydrogen peroxide in a solution and is decreased by the addition of catalase to the solution. The initial slope of the time-dependent current decrease after the addition of human blood cells to a test solution is directly proportional to the catalase activity. The catalase activity of normal human blood cells was measured as 4.53 mol/hr·ml<SUP>-1</SUP> by this method. This method has an advantage over the previously proposed methods using an oxygen electrode, whereby no deaeration of the test solution is required with the present method.
Japan Society of Clinical Chemistryの論文