A Simple and Sensitive Polymerase Chain Reaction Method for the Detection of Food-Related Bacteria
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概要
- 論文の詳細を見る
We devised a simple and efficient method of preparing chromosomal DNA from Gram-positive bacteria for use as a template in PCR, which consists of achromopeptidase treatment followed by Chelex 100 treatment. Using template DNA prepared by this method, it was possible to detect as few as one colony forming unit of some kinds of food-related bacteria using universal primers targeting the bacterial 16S ribosomal RNA gene or specific primers for bacterial genes under fixed thermocycling conditions.
- 社団法人 日本食品科学工学会の論文
- 2000-05-01
著者
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Yasokawa Daisuke
Hokkaido Food Processing Research Center, Department of Food Biotechnology
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Okumura Yukihiro
Hokkaido Food Processing Research Center, Department of Food Biotechnology
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Nagashima Koji
Hokkaido Food Processing Research Center
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Nakagawa Ryoji
Hokkaido Food Processing Research Center
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Yasokawa Daisuke
Hokkaido Food Processing Res. Center
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Nagashima Koji
Hokkaido Food Processing Res. Center
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Kawakami M
Hokkaido Food Processing Res. Center Ebetsu Jpn
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Nakagawa Ryoji
Section Of Biotechnology Department Of Applied Technology Hokkaido Food Processing Research Center
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KAWAKAMI Makoto
Hokkaido Food Processing Research Center
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SHIMIZU Takeshi
Hokkaido Food Processing Research Center
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TAKESHI Kouichi
Hokkaido Institute of Public Health
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Okumura Ko
山梨大学 医学部免疫学講座
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OKUMURA Yasushi
Applied Technology Research Laboratory Asahi Breweries Ltd
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Okumura Yukihiro
Hokkaido Food Processing Research Center Department Of Food Biotechnology
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Kawakami M
Hokkaido Food Processing Research Center
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Shimizu T
Section Of Biotechnology Department Of Applied Technology Hokkaido Food Processing Research Center:(
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OKUMURA Yukihiro
Hokkaido Food Processing Research Center
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