Cloning and Sequence Analysis of cDNA Coding for a Lectin from Helianthus tuberosus Callus and Its Jasmonate-induced Expression
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概要
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Two lectins (designated as HTA I and HTA II) that seemed to be isolectins were found in Helianthus tuberosus callus. cDNA encoding HTA I was isolated from a ZAP Express expression library by immunoselection by using the anti-HTA antiserum. The sequence of this cDNA consisted of 432bp nucleotides coding for a polypeptide of 143 amino acid residues (Mr, 15,314). When introduced into E.coli, the cDNA directed the synthesis of active HTA I as indicated by the hemagglutination activity. The deduced amino acid sequence showed homology with some lectins and jasmonate-induced proteins. When callus was cultured in the presence of methyl jasmonate (MeJA), the hemagglutination activity increased in a dose-dependent manner. The levels of expression of the HTA protein and of the corresponding mRNA also increased in the treated callus. In view of these results, HTA I is considered to be a jasmonate-induced protein.
- 社団法人日本農芸化学会の論文
- 2000-06-23
著者
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Yasokawa Daisuke
Hokkaido Food Processing Research Center, Department of Food Biotechnology
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Okumura Yukihiro
Hokkaido Food Processing Research Center, Department of Food Biotechnology
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Nagashima Koji
Hokkaido Food Processing Research Center
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Nakagawa Ryoji
Hokkaido Food Processing Research Center
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Yasokawa Daisuke
Hokkaido Food Processing Res. Center
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Nagashima Koji
Hokkaido Food Processing Res. Center
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Nakagawa Ryoji
Section Of Biotechnology Department Of Applied Technology Hokkaido Food Processing Research Center
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Okumura Ko
山梨大学 医学部免疫学講座
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OKUMURA Yasushi
Applied Technology Research Laboratory Asahi Breweries Ltd
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Okumura Yukihiro
Hokkaido Food Processing Research Center Department Of Food Biotechnology
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OKUMURA Yukihiro
Hokkaido Food Processing Research Center
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