Reactivities of Mutants of a Major House Dust Mite Allergen Der f 2 to Mouse Anti-Der f 2 Monoclonal Antibodies Analyzed by Immunoblotting

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概要

• 論文の詳細を見る

• A total of sixteen recombinant variants of a major house dust mite allergen Der f 2,the wild-type Der f 2,six cysteine mutants, six proline mutants, and three lysine mutants, were expressed in Escherichia coli. The cells were solubilized and run on SDS-PAGE under reducing conditions. Epitopes for five mouse anti-Der f 2 monoclonal antibodies, 1B2,7C10,13A4,15E11,and 18G8,to the recombinant Der f 2 variants were characterized by immunoblot analysis.

• 社団法人日本農芸化学会の論文
• 2001-03-23

著者

• Yokota T

  Foods & Pharmaceuticals Research & Development Laboratory Asahi Breweries Ltd.

• Tokura Tomoko

  Atopy (allergy) Research Center Juntendo University School Of Medicine

• Takai T

  Atopy (allergy) Research Center Juntendo University School Of Medicine

• TAKAI Toshiro

  Bioscience Research and Development Laboratory, Asahi Breweries, Ltd.

• OKUMURA Yasushi

  Bioscience Research and Development Laboratory, Asahi Breweries, Ltd.

• YOKOTA Toyokazu

  Bioscience Research and Development Laboratory, Asahi Breweries, Ltd.

• AKAGAWA-CHIHARA Midori

  Bioscience Research & Development Laboratory, Asahi Breweries, Ltd.

• Okumura Ko

  Atopy (allergy) Research Center Juntendo University School Of Medicine

• Okumura Ko

  山梨大学 医学部免疫学講座

• OKUMURA Yasushi

  Applied Technology Research Laboratory Asahi Breweries Ltd

• Yokota T

  Faculty Of Agriculture Shizuoka University

• Yuuki T

  Asahi Breweries Ltd. Ibaraki Jpn

• Akagawa-chihara Midori

  Bioscience Research And Development Laboratory Asahi Breweries Ltd.

• Takai Toshiro

  Atopy (allergy) Research Center

• Yokota Takao

  Iss Science Project Office Institute Of Space And Astronautical Science Japan Aerospace Exploration

• Takai Toshio

  Bioscience Research and Development Laboratory, Asahi Breweries, Ltd.

• Yokota Toyokazu

  Bioscience Research & Development Laboratory, Asahi Breweries, Ltd.

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