Cloning and Expression of cDNA Coding for the Major House Dust Mite Allergen Der f II in Escherichia coli(Biological Chemistry)

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概要

• 論文の詳細を見る

• A cDNA library corresponding to mite protein was screened using anti-Der f II, a major allergen from the house dust mite Dermatophagoides farinae, antibody. Three possible clones were obtained that contained cDNA fragments coding for Der f II, and the nucleotide sequences of the fragments were determined. There were minor differences observed affecting the deduced amino acid sequence among the three cDNA fragments. The amino acid sequence of the purified native Der f II protein could be analyzed to 45 residues from the N-terminus. As a result of comparison, all the three cDNA fragments code for a mature protein with a derived molecular weight of about 14,000. The amino acid sequence was not homologous to any known protein sequences and it contained six cysteine residues and no N-glycosylation sites.

• 社団法人日本農芸化学会の論文
• 1991-05-23

著者

• SUKO Matsunobu

  Department of Medicine and Physical Therapy, University of Tokyo, Faculty of Medicine

• OKUDAIRA Hirokazu

  Department of Medicine and Physical Therapy, University of Tokyo, Faculty of Medicine

• Suko Matsunobu

  Department Of Medicine And Physical Therapy University Of Tokyo Faculty Of Medicine

• Suko Matsunobu

  Department Of Medicine And Physical Therapy Faculty Of Medicine University Of Tokyo

• Ando Toru

  Research Laboratories Torii & Co. Ltd.

• OKUMURA Yasushi

  Central Research Laboratories, Asahi Breweries Ltd.

• YAMAKAWA Hiroshi

  Research Laboratories, Torii Pharmaceutical Co., Ltd.

• Okudaira Hirokazu

  Department Of Medicine And Physical Therapy Faculty Of Medicine The University Of Tokyo

• Okumura Ko

  山梨大学 医学部免疫学講座

• OKUMURA Yasushi

  Applied Technology Research Laboratory Asahi Breweries Ltd

• YUUKI Toshifumi

  Central Research Laboratories, Asahi Breweries, Ltd.

• HAIDA Michiko

  Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo

• Yuuki T

  Asahi Breweries Ltd. Ibaraki Jpn

• Haida Michiko

  Department Of Medicine And Physical Therapy Faculty Of Medicine University Of Tokyo

• Okudaira H

  Department Of Medicine And Physical Therapy Faculty Of Medicine University Of Tokyo

• Yuuki Toshifumi

  Central Research Laboratories Asahi Breweries Ltd.

• Okumura Yasushi

  Central Research Laboratories Asahi Breweries Ltd.

• Yamakawa Hiroshi

  Research Institute For Sustainable Humanosphere Kyoto University

• Yamakawa Hiroshi

  Research Laboratories Torii & Co. Ltd.

• Yokota Takao

  Iss Science Project Office Institute Of Space And Astronautical Science Japan Aerospace Exploration

• Haida Michiko

  Department of Internal Medicine, University of Tokyo Hospital, Japan

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