Effects of Protein Kinase Inhibitors on the Accumulation Kinetics of p53 Protein in Normal Human Embryo Cells following X-irradiation
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概要
- 論文の詳細を見る
DNA-damaging agents induce phosphorylation of the p53 protein, resulting in its accumulation in the nucleus. To clarify the signal transduction pathway(s) involved in p53 protein accumulation in normal human embryo cells following X-irradiation, the effects of three protein kinase inhibitors were examined. Quercetin, an inhibitor of heat-shock response, dose dependently suppressed the p53 accumulation induced by X-rays at more than 100μM. No suppression, however, was observed with calphostin-C, a specific inhibitor of protein kinase C, in the range of 0.05 to 0.25μM. Wortmannin was the most potent inhibitor of p53 accumulation. Its suppressive effect appears within a few minutes of pretreatment with a dose of 25μM, but posttreatment was less effective. Our findings suggest that PKC is not involved in X-ray-induced p53 accumulation in normal human embryo cells and that a wortmannin-sensitive pathway acts as a sensor of DNA damage.
- 日本放射線影響学会の論文
著者
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KODAMA SEIJI
Laboratory of Radiation and Life Science, Department of Health Sciences, School of Pharmaceutical Sc
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Ghosh Jagadish
Laboratory Of Radiation And Life Science Department Of Health Science School Of Pharmaceutical Scien
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Kodama Seiji
Division Of Radiation Biology And Health Science Research Center For Radiation And Radioisotopes Res
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SUZUKI KEIJI
Laboratory of Radiation and Life Science, Department of Health Science, School of Pharmaceutical Sci
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Watanabe Masami
Laboratory Of Plant Nutrition Faculty Of Horticulture Chiba University
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Suzuki K
Course Of Life Sciences And Radiation Research Graduate School Of Biomedical Sciences Nagasaki Unive
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Kodama Seiji
Laboratory Of Radiation And Life Science Department Of Health Sciences School Of Pharmaceutical Sciences Nagasaki University
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