Exogenous Expression of Exonuclease Domain-deleted WRN Interferes with the Repair of Radiation-induced DNA Damages

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概要

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• Werner syndrome (WS) is an autosomal recessive disease characterized by multiple progeroid features. The gene responsible for WS, WRN, is a member of the human RecQ helicase family. WRN is unique among this family, associated with an exonuclease activity. In the present study, we established the human 293-derived cell lines, which expressed exogenously truncated WRN protein, lacking the N-terminal exonuclease domain but having normal helicase activity, and found that they were slightly, but nonetheless significantly, radiosensitive than control cell lines, into which the empty vector had been introduced. The truncated WRN-expressing cells also exhibited increased numbers of micronuclei, chromosome aberrations, and the foci of phosphorylated histone H2AX with X-rays. These results suggested a function of WRN exonuclease activity that is separable from helicase activity and is essential for the repair of radiation-induced DNA damages.

• 日本放射線影響学会の論文
• 2005-12-28

著者

• Suzuki Keiji

  Course Of Life Sciences And Radiation Research Graduate School Of Biomedical Sciences Nagasaki Unive

• Suzuki Keiji

  大阪府立大学先端科学イノベーションセンター 放射線生命科学

• Suzuki Keiji

  Division Of Radiation Biology Department Of Radiology And Radiation Biology Graduate School Of Biome

• Kodama Seiji

  Radiation Biology Laboratory Radiation Research Center Frontier Science Innovation Center Organizati

• Kodama Seiji

  京都大学原子炉実験所 粒子線生物学研究分野

• Kodama Seiji

  長崎大学 医歯薬学総合研究科放射線生物学

• Watanabe Masami

  Particle Radiation Biology Research Reactor Institute Kyoto University

• WATANABE Masami

  Laboratory of Radiation Biology, Division of Radiation Life Science, Department of Radiation Life Sc

• Kodama S

  Radiation Biology Laboratory Radiation Research Center Frontier Science Innovation Center Organizati

• Kodama Seiji

  Division Of Radiation Biology And Health Science Research Center For Radiation And Radioisotopes Res

• Kashino Genro

  Particle Radiation Oncology Research Center Kyoto University

• Kashino Genro

  Particle Radiation Oncology Research Centre Research Reactor Institute Kyoto University

• Kashino Genro

  Division of Radiation Biology, Department of Radiology and Radiation Biology, Nagasaki University Gr

• MATSUMOTO Takehisa

  Genomic Sciences Center, Riken Yokohama Institute

• Watanabe Masani

  National Institute Of Basic Biology (nibb)

• Watanabe Masami

  Laboratory Of Plant Nutrition Faculty Of Horticulture Chiba University

• Watanabe Masami

  京都大学原子炉実験所 粒子線生物学研究分野

• Matsumoto Takehisa

  Genomic Sciences Center Riken Yokohama Institute

• Kashino Genro

  京都大学原子炉実験所附属粒子線腫瘍学研究センター

• Watanabe M

  Particle Radiation Biology Research Reactor Institute Kyoto University

• Kasai Soko

  Department Of Medicinal Chemistry Center For Frontier Research In Medicinal Science 21st Century Coe

• Kasai Soko

  Department Of Biological Science And Technology Faculty Of Engineering University Of Tokushima

• MATSUMOTO Takuro

  Nagoya Univ.

• Watanabe Masani

  Division Of Radiation Biology Faculty Of Pharmacology Of Science Nagasaki University

• Suzuki K

  Division Of Radiation Biology Department Of Radiology And Radiation Biology Course Of Life Sciences

• Kasai Soko

  Course Of Life Sciences And Radiation Research Graduate School Of Biomedical Sciences Nagasaki Unive

• Watanabe Masami

  Lab. Of Radiation Biology Div. Of Radiation Life Sci. Res. Reactor Inst. Kyoto Univ.

• Kashino Genro

  Particle Radiation Oncology Research Center Kyoto University Research Reactor Institute

• Suzuki Keiji

  Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Kashino Genrou

  Particle Radiation Oncology Research Centre Research Reactor Institute Kyoto University

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