Involvement of Reactive Oxygen Species (ROS) in the Induction of Genetic Instability by Radiation
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概要
- 論文の詳細を見る
Radiation generates reactive oxygen species (ROS) that interact with cellular molecules, including DNA, lipids, and proteins. To know how ROS contribute to the induction of genetic instability, we examined the effect of the anti-ROS condition, using both ascorbic acid phosphate (APM) treatment or a low oxygen condition, on the induction of delayed reproductive cell death and delayed chromosome aberrations. The primary surviving colonies of mouse m5S-derived cl. 2011-14 cells irradiated with 6 Gy of X-rays were replated and allowed to form secondary colonies. The anti-ROS treatments were applied to either preirradiation culture or postirradiation cultures for primary or secondary colony formation. Both anti-ROS conditions relieved X-ray-induced acute cell killing to a similar extent. These anti-ROS conditions also relieved genetic instability when those conditions were applied during primary colony formation. However, no effect was observed when the conditions were applied during preirradiation culture and secondary colony formation. We also demonstrated that the amounts of ROS in X-ray-irradiated cells rapidly increase and then decrease at 6 hr postirradiation, and the levels of ROS then gradually decrease to a baseline within 2 weeks. The APM treatment kept the ROS production at a lower level than an untreated control. These results suggest that the cause of genetic instability might be fixed by ROS during a 2-week postirradiation period.
- 日本放射線影響学会の論文
- 2004-06-15
著者
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Suzuki Keiji
Course Of Life Sciences And Radiation Research Graduate School Of Biomedical Sciences Nagasaki Unive
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Suzuki Keiji
大阪府立大学先端科学イノベーションセンター 放射線生命科学
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Suzuki Keiji
Division Of Radiation Biology Department Of Radiology And Radiation Biology Graduate School Of Biome
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Kodama Seiji
京都大学原子炉実験所 粒子線生物学研究分野
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Kodama Seiji
長崎大学 医歯薬学総合研究科放射線生物学
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Watanabe Masami
Particle Radiation Biology Research Reactor Institute Kyoto University
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Matsuda Naoki
Division Of Radiation Biology And Protection Center For Frontier Life Sciences Nagasaki University
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Kodama S
Radiation Biology Laboratory Radiation Research Center Frontier Science Innovation Center Organizati
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Kodama Seiji
Division Of Radiation Biology And Health Science Research Center For Radiation And Radioisotopes Res
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WATANABE Masami
Division of Radiation Biology, Department of Radiology and Radiation Biology, Course of Life Science
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TOMINAGA Hideyuki
Division of Radiation Biology, Department of Radiology and Radiation Biology, Graduate School of Bio
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MATSUDA Naoki
Center for Frontier Life Sciences, Division of Radiation Biology and Protection, Radioisotope Center
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Watanabe Masani
National Institute Of Basic Biology (nibb)
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Watanabe Masami
京都大学原子炉実験所 粒子線生物学研究分野
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Mukaida Naoki
Division Of Radiation Biology And Protection Center For Frontier Life Sciences Nagasaki University
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Tominaga Hideyuki
Division Of Radiation Biology Department Of Radiology And Radiation Biology Graduate School Of Biome
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Watanabe M
Particle Radiation Biology Research Reactor Institute Kyoto University
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Kasai Soko
Department Of Medicinal Chemistry Center For Frontier Research In Medicinal Science 21st Century Coe
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Kasai Soko
Department Of Biological Science And Technology Faculty Of Engineering University Of Tokushima
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Watanabe Masani
Division Of Radiation Biology Faculty Of Pharmacology Of Science Nagasaki University
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Suzuki K
Division Of Radiation Biology Department Of Radiology And Radiation Biology Course Of Life Sciences
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Kasai Soko
Course Of Life Sciences And Radiation Research Graduate School Of Biomedical Sciences Nagasaki Unive
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Watanabe Masami
Lab. Of Radiation Biology Div. Of Radiation Life Sci. Res. Reactor Inst. Kyoto Univ.
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Suzuki Keiji
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Watanabe Masami
Division Of Radiation Biology School Of Medicine Yokohama City University
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