Biochemical Markers for in Situ Detection of DNA Double Strand Breaks in Cultured Mammalian Cells
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概要
- 論文の詳細を見る
In vitro expansion of cells is often limited by stress-induced premature senescence, and DNA double strand breaks are the most relevant damage related to the irreversible cell cycle arrest caused by oxidative stress. Recently, it has been shown that DNA damaging checkpoint proteins are activated by phosphorylation at the sites of DNA double strand breaks caused by ionizing radiation. ATM protein, which is the product of the gene mutated in radiation sensitive disorder called ataxia-telangiectasia (AT), functions as the primary sensor for DNA double strand breaks, and when visualized with an antibody recognizing phosphorylated ATM at S1981, phosphorylated ATM appears as nuclear foci after irradiation. The foci are induced dose-dependently, and the number of phosphorylated ATM foci is well correlated with the estimated number of DNA double strand breaks, providing an unique and sensitive tool for the measurement of DNA double strand breaks at the physiological level in situ.
- 日本再生歯科医学会の論文
- 2005-12-30
著者
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Suzuki Keiji
Course Of Life Sciences And Radiation Research Graduate School Of Biomedical Sciences Nagasaki Unive
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Suzuki Keiji
大阪府立大学先端科学イノベーションセンター 放射線生命科学
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Suzuki Keiji
Division Of Radiation Biology Department Of Radiology And Radiation Biology Graduate School Of Biome
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Kodama Seiji
Radiation Biology Laboratory Radiation Research Center Frontier Science Innovation Center Organizati
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Kodama Seiji
京都大学原子炉実験所 粒子線生物学研究分野
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Kodama Seiji
長崎大学 医歯薬学総合研究科放射線生物学
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Watanabe Masami
Particle Radiation Biology Research Reactor Institute Kyoto University
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WATANABE Masami
Laboratory of Radiation Biology, Division of Radiation Life Science, Department of Radiation Life Sc
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Kodama S
Radiation Biology Laboratory Radiation Research Center Frontier Science Innovation Center Organizati
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Kodama Seiji
Division Of Radiation Biology And Health Science Research Center For Radiation And Radioisotopes Res
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Watanabe Masani
National Institute Of Basic Biology (nibb)
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Watanabe Masami
Laboratory Of Plant Nutrition Faculty Of Horticulture Chiba University
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Watanabe Masami
京都大学原子炉実験所 粒子線生物学研究分野
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Watanabe M
Particle Radiation Biology Research Reactor Institute Kyoto University
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Kasai Soko
Department Of Medicinal Chemistry Center For Frontier Research In Medicinal Science 21st Century Coe
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Kasai Soko
Department Of Biological Science And Technology Faculty Of Engineering University Of Tokushima
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Watanabe Masani
Division Of Radiation Biology Faculty Of Pharmacology Of Science Nagasaki University
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Suzuki K
Division Of Radiation Biology Department Of Radiology And Radiation Biology Course Of Life Sciences
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Kasai Soko
Course Of Life Sciences And Radiation Research Graduate School Of Biomedical Sciences Nagasaki Unive
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Watanabe Masami
Lab. Of Radiation Biology Div. Of Radiation Life Sci. Res. Reactor Inst. Kyoto Univ.
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Suzuki Keiji
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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