A Rapid and Highly Sensitive Method for Diagnosis of Equine Influenza by Antigen Detection Using Immuno-PCR

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概要

• 論文の詳細を見る

• A rapid and highly sensitive method for diagnosis of influenza by detecting viral antigens using imununo-PCR has been developed. The sensitivity of the immuno-PCR for the detection of the nonstructural protein 1 (NS 1) antigenically common among influenza A viruses was 10^<1.0～2.0> times higher than that of RT-PCR for the detection of the viral genome. For the detection of the hemagglutinin (HA) subtype-specific antigen, this assay using anti-HA monoclonal antibodies attained a sensitivity of up to 10^<7.0～8.0> times higher than those by virus isolation or by RT-PCR.

• 北海道大学の論文
• 2001-02-28

著者

• KIDA Hiroshi

  Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University

• OZAKI Hiroichi

  Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University

• Kida Hiroshi

  東京大 海洋研 大槌臨海研究セ

• Sugita Shigeo

  Division Of Molecular Biology Tochigi Laboratory Of Equine Research Institute Japan Racing Associati

• Kida Hiroshi

  Hokkaido Univ. Hokkaido Jpn

• Kida Hiroshi

  Department Of Cardiology Kanazawa National Hospital

• Kida Hiroshi

  Department Of Disease Control Graduate School Of Veterinary Medicine Hokkkaido University

• Ozaki Hiroichi

  Department Of Disease Control Graduate School Of Veterinary Medicine Hokkaido University

• Ozaki Hiroichi

  Department Of Disease Control Hokkaido University Graduate School Of Veterinary Medicine

• Kida Hiroshi

  Laboratory Of Microbiology Department Of Disease Control Graduate School Of Veterinary Medicine Hokk

• Kida Hiroshi

  Department Of Disease Control Graduate School Of Veterinary Medicine Hokkaido University

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