Effect of Sendai Virus Exposure on the Development of Frozen-Thawed Mouse Embryos

概要

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Frozen-thawed mouse preimplantation embryos were exposed to Sendai virus at the blastocyst and morula stages to determine if these embryos were susceptible to virus infection. It was also examined whether the frozen-thawed embryos would further develop into expanded blastocysts after <I>in vitro</I> culture. Approximately 50% of the frozen-thawed embryos at the blastocyst stage were susceptible to Sendai virus infection. On the other hand, frozen-thawed embryos exposed to Sendai virus at the morula stage were resistant to the infection. It was noted that most of the frozen-thawed embryos exposed to Sendai virus developed into expanded blastocysts. Significant differences in the development to expanded blastocysts were observed in the Sendai virus-exposed frozen-thawed blastocysts (57.0%) compared with the frozen-thawed control (82.1%) and unfrozen control (93.7%) blastocysts. However, development to expanded blastocysts of Sendai virus exposed frozen-thawed morulae (73.0%) was similar to the frozen-thawed control (81.0%) but significantly different with the unfrozen control (93.2%) morulae. These findings suggest that some changes in the structure of the zona pellucida may occur during freezing procedures making the frozen-thawed embryos susceptible to virus infection.
日本繁殖生物学会の論文