INDUCTIONS OF SUPEROVULATION USING SEVERAL FSH REGIMENS IN HOLSTEIN-FRIESIAN HEIFERS

概要

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Eighty-three Holstein-Friesian virgin heifers were randomly divided into four groups and superovulated with one of the four FSH regimens as follows : Group I) twice daily injections of a constant dose of 5 mg of FSH for a period of 4 days, giving a total dose of 40 mg in 8 fractions ; Group II) as in Group I with additional 5 mg of FSH on the 5th day, giving a total dose of 45 mg ; Group III) twice daily injections on a decreasing dose regimen of 5,4,3 and 2 mg successively for 4 days with the addition of 2 mg of FSH once on the 5th day, giving a total dose of 30 mg; Group IV) similar to Group III, but a mixture of FSH and LH in the ratio of 5 : 1 was used, giving a total dose of 30 mg of FSH and 6 mg of LH in 9 fractions. Nonsurgical embryo recovery was performed 7 or 8 days after estrus. Percentage of good embryos in Group II was significantly greater than that in Group I (P<0.05). There was no significant difference between Groups II and III in any of the criteria analyzed. Group IV was superior to the other groups in superovulation rate and embryo yielding. Significantly greater numbers of total eggs and good embryos were observed in Group IV compared to those in Groups I and II (P<0.05). Percentage of the heifers with more than 3 good embryos in Group IV was significantly higher than that in Groups I , II and III (P<0.05).
北海道大学の論文
1985-04-30