TIMING OF SEQUENTIAL CHANGES IN CHROMOSOME CONFIGURATIONS DURING THE 1st MEIOTIC DIVISION OF PIG OOCYTES CULTURED IN VITRO

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概要

• 論文の詳細を見る

• This study examines the timing of changes in chromosome configurations of pig oocytes derived from small antral follicles of follicular and/or inactive stage donors using modified 199 medium supplemented with gonadotropins (Follicle Stimulating Hormone (FSH), 10 IU/ml ; Human Chorionic Gonadotropin (hCG), 10 IU/ml) and Glucosamine (0.539mg/ml). Oocytes (n=1,215) were fixed at the end of 3 hourly intervals from 0-48 hr of culture. Results were expressed as the percentage of oocytes at each stage of maturation for each time point. The germinal vesicle (GV) stage was observed for the first 17.6 hr ; germinal vesicle breakdown (GVBD) stage between 17.6-26.4 hr ; metaphase I (M-I) from 26.4-30.9 hr ; anaphase I (A-I) ranged from 30.9-33.4 hr ; telophase I (T-I) at 33.4-34.4 hr ; and metaphase II (M-II) at 34.4-48 hr.

• 北海道大学の論文
• 1990-12-28

著者

• Ocampo Lerma

  Department Of Animal Science Faculty Of Agriculture Hokkaido University

• Ocampo Marlon

  Department Of Theriogenology Faculty Of Veterinary Medicine

• KANAGAWA Hiroshi

  Department of Theriogenology, Faculty of Veterinary Medicine, Hokkaido University

• Kanagawa Hiroshi

  Department Of Biomedical Science Ontario Veterinary College University Of Guelph

• Kanagawa Hiroshi

  Department Of Theriogenology Faculty Of Veterinary Medicine

• KANAGAWA Hiroshi

  Department of Theriogenology, Faculty of Veterinary Medicine

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